The HuProt™ human proteome microarray v4.0 contains >21,000 unique, newly resequenced and individually purified human proteins encompassing 16,793 genes (~81% of the canonical proteome – as defined by the Human Protein Atlas [www.proteinatlas.org/about/licence]) and 124 unique mouse gene symbols. Recombinant proteins are expressed in yeast (S. cerevisiae), purified and printed on glass slides in duplicate, along with control proteins.

After expression, the N-terminal GST- and His6-tagged proteins are purified and printed on glass slides in duplicate, along with control proteins (GST, BSA, Histones, IgG, etc.) and new controls that allow for the use of linear regression analysis, radio-labeled sample analysis and the use of different analytical software.

Slides are bar-coded for tracking/archiving. Each microarray batch is routinely evaluated by GST immunoblotting (98% of all proteins authenticate to their respective molecular weights).

The HuProt human proteome microarray can be used for many applications:

  • Antibody Specificity
  • Serum/Biomarker Profiling
  • Small Molecule Profiling
  • Protein-Protein Interaction
  • DNA/RNA Binding
  • Enzyme Substrate ID
CDI HuProt™ microarray comprising GST fusion proteins probed with anti-GST antibody.
Functional categories of proteins contained in HuProt.

Antibody specificity profiling using the HuProt human proteome microarray.