“Retinal progenitor cells undergo a series of temporal transitions during neurogenesis. Many of the mechanisms controlling these processes are still largely unknown. Based on our previous single cell RNA sequencing studies profiling the full course of retinal neurogenesis, the Nuclear Factor I (NFI) transcription factors were found to be enriched in late stage progenitors. The Nfia/Nfib/Nfix conditional knockout mice have altered retinal morphology with a reduction in size of the inner nuclear layer which corresponds to a significant decrease in Müller glial cells and bipolar cells. The loss of the Nfigenes resulted in prolonged progenitor cell proliferation and increased photoreceptor specification. We also seek to identify the genes and transcriptional networks regulated by the NFI factors that are involved in cell fate specification. We used single cell RNA sequencing to profile over 80,000 cells at different developmental ages and ATAC sequencing to probe changes in transcription and chromatin accessibility in the absence of the NFI factors.  Using bioinformatic analyses we have identified genes and transcriptional networks that are regulated by Nfia, Nfib and Nfix  that correlate with cell-cycle exit and late born retinal cells.”