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Blackshaw Lab

Building and Rebuilding the Retina and Hypothalamus

  • Home
  • Research
  • Publications
  • Members
  • Datasets & Techniques
  • Home
  • Research
  • Publications
  • Members
  • Datasets & Techniques

Category: <span>Protocol</span>

Protein microarray-based profiling of antibody specificity

Antibody specificity, Protocol

Ramos-Lopez P, Irizarry J, Pino I and Blackshaw S. Methods in Molecular Biology, 2018, 1785:223-229.

Link to Article>>

In vivo electroporation of developing mouse retina

In vivo electroporation, Protocol

de Melo J and Blackshaw S. Retinal Gene Therapy, in Methods in Molecular Biology, 2018, 1715:101-111.

Link to Article>> View PDF>>

Identification of SUMO E3 Ligase Specific Substrates Using Human Proteome Microarrays

Protein microarray, Proteomics, Protocol, SUMOylation

Cox E, Uzoma I, Guzzo CM, Jeong JS, Matunis M, Blackshaw S and Zhu H. Methods Mol Biol 2015 295:455-63.

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Large-scale in situ hybridization in developing mouse diencephalon

Protocol, Transcriptomics

Shimogori T and Blackshaw S. Neuromethods 2015 99:307-220.

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High-throughput in situ hybridization in mouse retina

Protocol, Transcriptomics

Blackshaw S. 2013 Methods Mol Biol 935:215-26.

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Probing the functional role of adult hypothalamic neurogenesis by computer tomography image guided focal irradiation

Hypothalamus, Neurogenesis, Protocol

Lee DA, Salvatierra J, Velarde E, Wong J, Ford E, Blackshaw S. JoVE 2013 14: 10.379/50716.

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In vivo electroporation of neonatal mouse retina

Protocol

De Melo J and Blackshaw S. JoVE 2011 pi: 2847

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Characterization of protein-DNA interactions using protein microarrays

Protein microarray, Protocol

Hu S, Xie Z, Blackshaw S, Qian J, Zhu H. Cold Spring Harb Protoc, 2011 pii.pdb.prot5614.

Link to Article>> View PDF>>

Serial Analysis of Gene Expression

Protocol, SAGE

Blackshaw S, Kim JB, St. Croix B, Polyak K. Current Protocols in Human Genetics, Unit 11.6 2007.

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Serial Analysis of Gene Expression

Protocol, SAGE

Blackshaw S, Kim JB, St. Croix B, Polyak K. Current Protocols in Molecular Biology, Unit 25B.6 2007.

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Research Areas

  • AAV
  • Antibody specificity
  • ATAC-Seq
  • Autoimmune disease
  • Behavior
  • Book Chapter
  • Cell fate
  • Cell lineage analysis
  • Chemical senses
  • Chemogenetics
  • ChIP-Seq
  • Circadian
  • Commentary
  • D-Amino acids
  • Development
  • Diabetes
  • Extraretinal photoreceptors
  • FGF
  • Human genetics
  • Hypothalamus
  • In vivo electroporation
  • Lens
  • Lhx2
  • lncRNAs
  • Metabolism
  • Muller glia
  • Neural circuitry
  • Neural development
  • Neurodegeneration
  • Neurogenesis
  • Neurotransmitters
  • New technique
  • Nitric oxide
  • Notch/Delta
  • Nucleogenesis
  • Photoreceptor
  • Protein microarray
  • Proteomics
  • Protocol
  • Radial glia
  • Retina
  • Review article
  • RNA binding proteins
  • RNA splicing
  • RPE
  • SAGE
  • scRNA-Seq
  • Shh
  • Signal transduction
  • Sleep
  • Spatial patterning
  • SUMOylation
  • Suprachiasmatic nucleus
  • Tanycytes
  • Thalamus
  • Transcription factor
  • Transcriptomics
  • Visual cycle
  • Wnt

Recent Posts

  • Cell-specific regulation of gene expression using splicing-dependent frameshifting
  • Ectopic insert-dependent neuronal expression of GFAP promoter-driven AAV constructs in adult mouse retina
  • LRLoop: A method to predict feedback loops in cell-cell communication
  • Notch Inhibition Promotes Regeneration and Immunosuppression Supports Cone Survival in a Zebrafish Model of Inherited Retinal Dystrophy
  • Genetic loss of function of Ptbp1 does not induce glia-to-neuron conversion in retina

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