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Blackshaw Lab

Building and Rebuilding the Retina and Hypothalamus

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The long noncoding RNA NEAT1 modulates neuronal excitability

lncRNAs, Protein microarray, Proteomics

Barry G, Briggs JA, Hwang DW, Nayler SP, Fortuna PRJ, Jonkhout N, Dachet F, Maag JLV, Mestdagh P, Singh EM, Avesson L, Kaczorowski DC, Ozturk E, Jones NC, Vetter I, Arriola-Martinez L, Hu J, Franco GR, Warn VM, Gong A, Dinger ME, Rigo F, Lipovich L, Morris MJ, O’Brien TJ, Lee DS, Loeb JA, Blackshaw S, Mattick JA, and Wolvetang EJ. Sci Reports 2017 7:40127.

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An Lhx1-Regulated Transcriptional Network Controls Sleep-Wake Coupling and Thermal Resistance of the Central Circadian Clockworks

Circadian, Hypothalamus, Sleep

Bedont JL, LeGates TA, Buhr E, Bathini A, Ling J, Wong P, van Gelder R, Mongrain V, Hattar S, and Blackshaw S. Curr Biol 2017 27:128-136.

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Understanding the role of lncRNAs in neuronal development

Neural development, RNA splicingneural development, RNA splicing

Clark BS and Blackshaw S. Long Noncoding RNA Biology, Springer Advances in Experimental Biology and Medicine, 2017;1008:253-28.

The stage-dependent roles of Ldb1 and functional redundancy with Ldb2 in mammalian retinogenesis

Cell fate, Development, Lhx2, Neurogenesis, Retina

Gueta K, Cohen T, David A, Nobel H, Narkis G, Li L, Love P, de Melo J, Blackshaw S, Westphal H, and Ashery-Padan R. Development 2016 143:4182-4192.

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The NIH Protein Capture Reagents Program (PCRP): a standardized affinity reagent toolbox

Antibody specificity, New technique, Protein microarray

Blackshaw S, Anderson S, Campbell E, Freeman N, Gatlin C, Pino I, Zhu H and Bader J. Nat Methods 2016 13:805-6.

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Identification of the PARP-1 dependent AIF-associated nuclease

Neurodegeneration

Wang Y, An R, Umanah GK, Nambiar K, Park H, Eaker SM, Kim BW, Harraz MM, Chang C, Chen R, Kam TI, Jeong JS, Xie Z, Neifert S, Qian J, Blackshaw S, Zhu H, Song H, Ming GL, Dawson VL, and Dawson TM. Science 2016 354: pii: aad6872.

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Control of lens development by Lhx2- regulated neuroretinal FGFs

FGF, Lens, Lhx2, Retina

Thein T, de Melo J, Zibetti C, Clark BS, Juarez F, and Blackshaw S. Development 2016 143: 3994-4002.

Link to Article>>

Multiple intrinsic factors act in concert with Lhx2 to direct retinal gliogenesis

Cell fate, Development, Lhx2, Muller glia, Neurogenesis, Notch/Delta, Radial glia, Retina, Signal transduction, Transcription factor

De Melo J, Clark BS, and Blackshaw S. Sci Reports 2016 6:32757.

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A trisomic potentiator of a disomic modifier of congenital heart disease penetrance in a mouse model of Down syndrome

Protein microarray, Proteomics

Li H, Edie S, Klinedinst D, Jeong JS, Blackshaw S, Maslen CL, and Reeves RH. Genetics 2016 203:763-7.

Link to Article>> View PDF>>

The nutrient sensor OGT in PVN neurons regulates feeding

Hypothalamus, Metabolism

Lagerlöf O, Slocomb JE, Hong I, Aponte Y, Blackshaw S, Hart GW and Huganir RL. Science 2016 351:1293-6.

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Research Areas

  • AAV
  • Antibody specificity
  • ATAC-Seq
  • Autoimmune disease
  • Behavior
  • Book Chapter
  • Cell fate
  • Cell lineage analysis
  • Chemical senses
  • Chemogenetics
  • ChIP-Seq
  • Circadian
  • Commentary
  • D-Amino acids
  • Development
  • Diabetes
  • Extraretinal photoreceptors
  • FGF
  • Human genetics
  • Hypothalamus
  • In vivo electroporation
  • Lens
  • Lhx2
  • lncRNAs
  • Metabolism
  • Muller glia
  • Neural circuitry
  • Neural development
  • Neurodegeneration
  • Neurogenesis
  • Neurotransmitters
  • New technique
  • Nitric oxide
  • Notch/Delta
  • Nucleogenesis
  • Photoreceptor
  • Protein microarray
  • Proteomics
  • Protocol
  • Radial glia
  • Retina
  • Review article
  • RNA binding proteins
  • RNA splicing
  • RPE
  • SAGE
  • scRNA-Seq
  • Shh
  • Signal transduction
  • Sleep
  • Spatial patterning
  • SUMOylation
  • Suprachiasmatic nucleus
  • Tanycytes
  • Thalamus
  • Transcription factor
  • Transcriptomics
  • Visual cycle
  • Wnt

Recent Posts

  • Cell-specific regulation of gene expression using splicing-dependent frameshifting
  • Ectopic insert-dependent neuronal expression of GFAP promoter-driven AAV constructs in adult mouse retina
  • LRLoop: A method to predict feedback loops in cell-cell communication
  • Notch Inhibition Promotes Regeneration and Immunosuppression Supports Cone Survival in a Zebrafish Model of Inherited Retinal Dystrophy
  • Genetic loss of function of Ptbp1 does not induce glia-to-neuron conversion in retina

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